Learn to maximize the performance of your HPLC or UHPLC system with this resource from leading experts in the field Optimization in HPLC: Concepts and Strategies delivers tried-and-tested strategies for optimizing the performance of HPLC and UHPLC systems for a wide variety of analytical tasks. The book explains how to optimize the different HPLC operation modes for a range of analyses, including small molecules, chiral substances, and biomolecules. It also shows readers when and how computational tools may be used to optimize performance. The practice-oriented text describes common challenges faced by users and developers of HPLC and UHPLC systems, as well as how those challenges can be overcome. Written for first-time and experienced users of HPLC technology and keeping pace with recent developments in HPLC instrumentation and operation modes, this comprehensive guide leaves few questions unanswered. Readers will also benefit from the inclusion of: A thorough introduction to optimization strategies for different modes and uses of HPLC, including working under regulatory constraints An exploration of computer aided HPLC optimization, including ChromSwordAuto and Fusion QbD A treatment of current challenges for HPLC users in industry as well as large and small analytical service providers Discussions of current challenges for HPLC equipment suppliers Tailor-made for analytical chemists, chromatographers, pharmacologists, toxicologists, and lab technicians, Optimization in HPLC: Concepts and Strategies will also earn a place on the shelves of analytical laboratories in academia and industry who seek a one-stop reference for optimizing the performance of HPLC systems.

Dieses handliche Fachbuch bietet HLPC-Experten ausfuhrliche praktische Informationen, um Gradienten-Trennverfahren zuverlassig und effizient durchzufuhren.

How can I use my HPLC/UHPLC equipment in an optimal way, where are the limitations of the technique? These questions are discussed in detail in the sequel of the successful "HPLC Expert" in twelve chapters written by experts in the respective fields. The topics encompass - complementary to the first volume - typical HPLC users' problems and questions such as gradient optimization and hyphenated techniques (LC-MS). An important key aspect of the book is UHPLC: For which analytical problem is it essential, what should be considered? Besides presentation of latest developments directly from the main manufacturers, also UHPLC users and independent service engineers impart their knowledge. Consistent with the target groups, the level is advanced, but the emphasis is on practical applications.

Filling the gap for an expert text dealing exclusively with the practical aspects of HPLC-MS coupling, this concise, compact, and clear book provides detailed information to enable users to employ the method most efficiently. Following an overview of the current state of HPLC-MS and its instrumentation, the text goes on to discuss all relevant aspects of method development. A chapter on tips and tricks is followed by user reports on the advantages - and pitfalls - of applying the method in real-life scenarios. The whole is rounded off by a look at future developments by renowned manufacturers.

The rapid development of HPLC instrumentation and technology opens numerous possibilities - and entails new questions. Which column should I choose to obtain best results, which gradient fits to my analytical problem, what are recent and promising trends in detection techniques, what is state of the art regarding LC-MS coupling? All these questions are answered by experts in ten self-contained chapters. Besides these more hardware-related and technical chapters, further related areas of interest are covered: Comparison of recent chromatographic data systems and integration strategies, smart documentation, efficient information search in internet, and tips for a successful FDA inspection. This practical approach offers in a condensed manner recent trends and hints, and will also display the advanced reader mistakes and errors he was not aware of so far.

Aus einem Verfahren fA1/4r Spezialisten in der Forschung hat sich die LC/MS-Kopplung zu einer bewAhrten Routineanwendung entwickelt. Ziel des Buchs ist, LC/MS-Anwendern mAglichst detaillierte Informationen zu geben, damit sie ihre LC/MS-Applikation optimal durchfA1/4hren kAnnen. Nach einem A berblick A1/4ber den gegenwArtigen Stand der Technik und instrumentelle Voraussetzungen werden Aspekte der Methodenentwicklung diskutiert. Ein Kapitel mit Tipps und Tricks leitet zu Anwenderberichte A1/4ber, bei denen auch ein Blick A1/4ber die klassische HPLC hinaus zur Ionenchromatographie geworfen wird. Berichte von renommierten GerAteherstellern A1/4ber neueste Entwicklungen runden das Buch ab. Dieses Praktikerbuch liefert LC/MS-Anwendern die Informationen, die sie zur optimalen Nutzung ihrer GerAte benAtigen: Von instrumentellen Voraussetzungen und Methodenentwicklung bis zu Anwender- und Herstellerberichten werden wichtige Themen kompakt auf den Punkt gebracht.

Beide ExpertenbA1/4cher im Set: Wie ntuze ich als erfahrener (U)HPLC-Anwender meine Anlage optimal? Welche MAglichkeiten und Grenzen haben Gradienten-HPLC, 2D-Chromatographie, LC-MS-Kopplung? Hard- und Software werden von Spezialisten auf den Punkt gebracht und erArtert.